The deadly wolves became extinct about 12,500 years ago. Now, thanks to genetic engineering, they have returned to what scientists described as “the first removal in the world.”
Scientists at Colossal Biosciences, a biotechnology -based conservation company famous for trying to do so RestoreSay that they succeeded in producing three evil wolf (Linoosion DerosPutting. They did this by rebuilding the wolf genome from the old DNADetermining the genes versions that were seen only in terrible wolves and not in relatives of extinct meat.
Then the team modified the DNA from the modern gray wolves (SLIS) To include these unique genetic variables. They inserted the DNA into the cells of the marble gray wolf eggs and allowed them to press the laboratory before inserting them into the uterus of alternative dogs, Time mentioned. Three embryos were successfully planted in three alternative dogs, which led to the birth of the snowy eggs called Romulus, Remus and khaleesi, which reminds us of Ghost, Jon Snow’s Dire Wolf in HBO’s.game of thrones“The TV series.
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“Our team got the 13,000 -year -old DNA and a 72,000 -year -old skull and has caused a terrible Wolf,” Bin Lam, CEO of Holosal said in a statement. “It has been said once,” any technique is advanced enough that cannot be distinguished from magic. “Today, our team gets to reveal some of the magic they are working on.”
How to make a terrible wolf
To “De-ExcTRICT” Dolly sheep In 1996.
To produce a perfect cloning with this method, one cell is removed from the subject of cloning and its genetic materials, which are kept in the cell nucleus. Then this genetic information is inserted into an egg of a giving animal of the same type that has its own nucleus. The egg now contains all the genetic information required to rebuild the theme of the original cloning and is transferred to an alternative to pregnancy for the duration.
However, since the terrible wolves were extinct at the end of the last ice age, Colossal had to bring their terrible wolf cells to collect by gathering evidence from the fossil record.
To rebuild the animal’s genome, they used genetic samples of severe wolf fossils and compared these genomics to their living relatives, including wolves, affiliations and foxes. They chose gray wolves to be donated with eggs, because these animals are the closest cousin of wolves.
This genetic analysis enabled scientists to determine 20 major differences in 14 genes that were responsible for the characteristics of severe wolves, including their larger size, white coat, larger teeth, and distinctive howl.
After that, they collected cells from blood samples from live gray wolves – a less invasive method than using a large part of the tissues, as happened in Dolly sheep. Then, using Crisper Genetic liberalization technology, the team made 20 modifications to the genes that were identified 14 to withstand the same sequence of relatives of the old wolves.
Once you create these “terrible wolf cells”, their nuclei was extracted and inserted into eggs from a living gray wolf. As with Dolly sheep, these donor egg cells had removed their own core to provide space for the new DNA. Reproductive cells – such as eggs and sperm – usually have only one copy of each chromosome; However, the cells of the body carry two copies. So the transfer of the nucleus from the body cell to a genitals provides it with all the genetic instructions necessary to mature in a fetus.
The eggs were allowed to ripen to embryos in the laboratory, which led to 45 fetuses that were transferred to the uterus of home dogs, technically, a sub -type of wolf.
Only one gene in each alternative is taken. After 65 days of pregnancy, Romulus and Remos were born across the Caesarean section, on October 1, 2024.
Colossal shared footage of Romulus and Remus while taking their first steps, making their first howl and developing newborns to the cubs of youth. They described terrible wolves as “the first removal in the world.”
However, there was another one of the persuasion: In 2003, scientists in Spain were reproduced in the types of extinct wild goats known as Boukardo, or Pix Perinan (Kabra Berenika Berenica). But the new goats died just minutes after birth, according to 2009 study.
The company said in a statement that Colossal has also announced the birth of players from cloned red wolves, which is the most vulnerable wolf in the world.
This advertisement comes a few months after the unveiled COLOSSAL The genetically engineered “woolen mice”And that has a similar fur for the thick hair of the Sufi mammoth, which represents a milestone in the company’s project to These titles prepared the era of ice by 2028.